Dallaire, F, Schreiner, S, Blair, GE orcid.org/0000-0003-0440-1795 et al. (3 more authors) (2016) The human adenovirus type 5 E4orf6/E1B55K E3 ubiquitin ligase complex can mimic E1A effects on E2F. mSphere, 1 (1).
Abstract
The human adenovirus E4orf6/E1B55K E3 ubiquitin ligase is well known to promote viral replication by degrading an increasing number of cellular proteins that inhibit the efficient production of viral progeny. We report here a new function of the Ad5 viral ligase complex that, although at lower levels, mimics effects of E1A products on E2F transcription factors. When expressed in the absence of E1A, the E4orf6 protein in complex with E1B55K binds E2F, disrupts E2F/Rb complexes and induces hyperphosphorylation of Rb, leading to induction of viral and cellular DNA synthesis as well as stimulation of early and late viral gene expression and production of viral progeny of E1/E3-defective adenovirus vectors. These new and previously undescribed functions of the E4orf6/E1B55K E3 ubiquitin ligase could play an important role in promoting the replication of wild-type viruses.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Editors: |
|
Copyright, Publisher and Additional Information: | (c) 2015, Dallaire et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
Keywords: | adenovirus; ubiquitin ligase; E4orf6; E1B55K; E1A |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 30 Oct 2015 10:50 |
Last Modified: | 23 Jun 2023 21:54 |
Published Version: | http://doi.org/10.1128/mSphere.00014-15 |
Status: | Published |
Publisher: | American Society for Microbiology |
Identification Number: | 10.1128/mSphere.00014-15 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:91298 |