Palmer, S.E., Smaczynska-de Rooij, I.I., Marklew, C.J. et al. (4 more authors) (2015) A Charge Swap mutation E461K in the yeast dynamin Vps1 reduces endocytic invagination. Communicative and Integrative Biology, 8 (4). e1051274. ISSN 1942-0889
Abstract
Vps1 is the yeast dynamin-like protein that functions during several membrane trafficking events including traffic from Golgi to vacuole, endosomal recycling and endocytosis. Vps1 can also function in peroxisomal fission indicating that its ability to drive membrane fission is relatively promiscuous. It has been of interest therefore that several mutations have been identified in Vps1 that only disrupt its endocytic function. Most recently, disruption of the interaction with actin through mutation of residues in one of the central stalk α helices (RR457,458 EE) has been shown to disrupt endocytosis and cause an accumulation of highly elongated invaginations in cells. This data supports the idea that an interaction between Vps1 and actin is important to drive the scission stage in endocytosis. Another Vps1 mutant generated in the study was vps1 E461K. Here we show data demonstrating that the E461K mutation also disrupts endocytosis but at an early stage, resulting in inhibition of the invagination step itself.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 The Author(s). Published with license by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/ 3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named a uthor(s) have been asserted. |
Keywords: | dynamin; dynamin-like protein; endocytosis; membrane trafficking |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 05 Nov 2015 17:23 |
Last Modified: | 05 Nov 2015 17:30 |
Published Version: | http://dx.doi.org/10.1080/19420889.2015.1051274 |
Status: | Published |
Publisher: | Taylor & Francis Open |
Refereed: | Yes |
Identification Number: | 10.1080/19420889.2015.1051274 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:91234 |