Warrander, Fiona, Faas, Laura, Kovalevskiy, Oleg et al. (5 more authors) (2015) lin28 proteins promote expression of 17∼92 family miRNAs during amphibian development. Developmental Dynamics. ISSN 1058-8388
Abstract
BACKGROUND: Lin28 proteins are post-transcriptional regulators of gene expression with multiple roles in development and the regulation of pluripotency in stem cells. Much attention has focussed on Lin28 proteins as negative regulators of let-7 miRNA biogenesis; a function that is conserved in several animal groups and in multiple processes. However, there is increasing evidence that Lin28 proteins have additional roles, distinct from regulation of let-7 abundance. We have previously demonstrated that lin28 proteins have functions associated with the regulation of early cell lineage specification in Xenopus embryos, independent of a lin28/let-7 regulatory axis. However, the nature of lin28 targets in Xenopus development remains obscure. RESULTS: Here we show that mir-17∼92 and mir-106∼363 cluster miRNAs are down regulated in response to lin28 knockdown, and RNAs from these clusters are co-expressed with lin28 genes during germ layer specification. Mature miRNAs derived from pre-mir-363 are most sensitive to lin28 inhibition. We demonstrate that lin28a binds to the terminal loop of pre-mir-363 with an affinity similar to that of let-7, and that this high affinity interaction requires to conserved a GGAG motif. CONCLUSION: Our data suggest a novel function for amphibian lin28 proteins as positive regulators of mir-17∼92 family miRNAs. This article is protected by copyright. All rights reserved.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2015 Wiley. This is an author produced version of a paper published in Developmental Dynamics. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/H000925/1 |
Depositing User: | Pure (York) |
Date Deposited: | 28 Oct 2015 11:36 |
Last Modified: | 05 Jan 2025 00:10 |
Published Version: | https://doi.org/10.1002/dvdy.24358 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1002/dvdy.24358 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:90985 |
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