Scherer, K.M., Bisby, R.H., Botchway, S.W. et al. (3 more authors) (2015) Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation. Journal of Biomedical Optics , 20 (7). 078003 . ISSN 1083-3668
Abstract
The uptake of E-combretastatins, potential prodrugs of the anticancer Z-isomers, into multicellular spheroids has been imaged by intrinsic fluorescence in three dimensions using two-photon excited fluorescence lifetime imaging with 625-nm ultrafast femtosecond laser pulses. Uptake is initially observed at the spheroid periphery but extends to the spheroid core within 30 min. Using agarose gels as a three-dimensional model, the conversion of Z(trans)→E(cis) via two-photon photoisomerization is demonstrated and the location of this photochemical process may be precisely selected within the micron scale in all three dimensions at depths up to almost 2 mm. We discuss these results for enhanced tissue penetration at longer near-infrared wavelengths for cancer therapy and up to three-photon excitation and imaging using 930-nm laser pulses with suitable combretastatin analogs.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/). Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
Keywords: | multiphoton; combretastatin; spheroid; fluorescence; imaging |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 07 Oct 2015 15:32 |
Last Modified: | 07 Oct 2015 15:32 |
Published Version: | http://dx.doi.org/10.1117/1.JBO.20.7.078003 |
Status: | Published |
Publisher: | SPIE |
Refereed: | Yes |
Identification Number: | 10.1117/1.JBO.20.7.078003 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:90584 |