Romero-Gonzalez, M.E. and Elayatt, A. (2015) Effect of Different Carbon Concentration on Yield of Extracellular Polymeric Substances (EPS) Produced by Pseudomonas Putida ATCC 11172. International Journal for Pharmaceutical Research Scholars (IJPRS), 4 (2). 475 - 481. ISSN 2277-7873
Abstract
The extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different carbon concentration, where Luria broth used as medium growth with and without addition of 0.5% w/v (LBG 0.5%) and 1.0% w/v (LBG 1%) glucose. The Bound EPS were extracted by ethylene diamine tetra acetic acid (EDTA) methods and precipitated by cold ethanol 3:1; while free EPS extracted only by precipitate by cold ethanol 3:1. The study shows the clear effect of carbon concentration on the production of EPS. The amount of free EPS found 120.39±2.20, 111.16±1.75 and 100.38±2.0 mg g-1 dry cell for LB, LBG 0.5 and LBG 1 respectively. Where the yield of bound EPS shows fewer amounts from free EPS, the amount of bound EPS was 29.22±1.40, 21.18±0.90 and 17.10±1.10 mg g-1 dry cell for LB, LBG 0.5 and LBG 1 respectively.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 IJPRS. This is an Open Access article distributed under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/3.0/) |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Civil and Structural Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 02 Oct 2015 15:08 |
Last Modified: | 02 Oct 2015 15:08 |
Published Version: | http://ijprs.com/abstract.php?id=671&aid=702 |
Status: | Published |
Publisher: | International Journal for Pharmaceutical Research Scholars (IJPRS) |
Refereed: | Yes |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:90255 |