Ponchel, F orcid.org/0000-0002-3969-7701, Burska, AN orcid.org/0000-0003-3321-9087, Hensor, EMA orcid.org/0000-0002-5245-4755 et al. (4 more authors) (2015) Changes in peripheral blood immune cell composition in osteoarthritis. Osteoarthritis and Cartilage, 23 (11). pp. 1870-1878. ISSN 1063-4584
Abstract
Objectives: Immune age-related abnormalities may synergise with osteoarthritis (OA) pathology. We explored whether abnormalities in the blood immune cell composition are present in OA, beyond defects typically associated with ageing. Design: Blood was collected from 121 healthy controls (HC) and 114 OA patients. Synovial biopsies were obtained from another 52 OA patients. Flow cytometry was used to establish the frequencies of lineage subsets, naïve, memory and regulatory T and B-cells, cells with an abnormal phenotype related to inflammation (IRC) and memory-like CD8+ T-cells. Multivariate analysis of covariance (MANCOVA) was used to determine whether the relative subset frequencies differed between HC and OA, controlling for age.
Results: Expected histology and T/B-cell infiltration were observed.
Following age adjusted analysis, we confirmed the lack of age association in HC for CD4+, B, NK and NKT cells but a negative trend for CD8+ T-cells. In OA, CD4+ T-cell and B-cell frequency were lower compared to HC while CD8+ T-cell frequencies were higher. CD8+ memory-like cells were more likely to be found in OA (odds ratio = 15). Increased CD8+ IRC frequencies were also present in OA. The relationship between age and CD4+ or CD8+ naïve T-cells in HC were changed in OA while the age relationships with memory cells were lost. The increase in CD4+ Treg with age was also lost in OA. B-cells showed limited evidence of disturbance. Conclusions: Immune dysfunction may be present in OA beyond what appears related to ageing; this requires further investigation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015, The Authors. Published by Elsevier Ltd and Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | OA; blood cell composition; cell subsets/phenotype; ageing |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Clinical Musculoskeletal Medicine (LIRMM) (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Inflammatory Arthritis (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Musculoskeletal Medicine & Imaging (Leeds) |
Funding Information: | Funder Grant number Versus Arthritis (formerly ARUK) 201494 NIHR National Inst Health Research NONE GIVEN |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 Sep 2015 14:10 |
Last Modified: | 16 Apr 2021 15:22 |
Published Version: | http://dx.doi.org/10.1016/j.joca.2015.06.018 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.joca.2015.06.018 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:89965 |