Stevens, JS, de Luca, AC, Downes, S et al. (2 more authors) (2014) Immobilisation of cell-binding peptides on poly-epsilon-caprolactone (PCL) films: A comparative XPS study of two chemical surface functionalisation methods. Surface and Interface Analysis, 46 (10-11). 673 - 678. ISSN 0142-2421
Abstract
Successful interaction between cells and biomaterial surfaces is crucial for biomedical devices, and incorporation of peptides such as RGD (Arg-Gly-Asp) at the polymer interface can substantially promote cell adhesion and proliferation. X-ray photoelectron spectroscopy (XPS) has been used to characterise poly-ε-caprolactone (PCL) films modified by aminolysis and the introduction of RGD peptides via carbodiimide (CDI) and thiol-halogen ‘click’ chemistry. The nitrogen signal acts as an elemental indicator for successful attachment, and changes in the chemical environment are reflected in the carbon and oxygen spectra. Chlorine and sulfur provide additional chemical indicators of reaction progress in the thiol method, with the selective nature of the Cl–S reaction reflected in the complete loss of Cl signal and appearance of S, avoiding potential amine-peptide side-reactions. Comparison of the XPS elemental concentrations indicated an estimate of 2–3% peptide functionalisation on the PCL surface for both methods, correlating with the improvement in Schwann cell response observed after peptide immobilisation. The enhanced selectivity of peptide attachment to the polymer surface demonstrated with XPS for the novel method based on thiol chemistry shows its potential for development as a biomimetic scaffold for peripheral nerve injury.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is the peer reviewed version of the following article: Stevens, JS, de Luca, AC, Downes, S, Terenghi, G and Schroeder, SLM (2014) Immobilisation of cell-binding peptides on poly-epsilon-caprolactone (PCL) films: A comparative XPS study of two chemical surface functionalisation methods. Surface and Interface Analysis, 46 (10-11). 673 - 678, which has been published in final form at http://dx.doi.org/10.1002/sia.5396. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
Keywords: | polymer; surface modification; substrate; RGD; biomaterial; scaffolds; cell response; X-ray photoelectron spectroscopy |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 12 Nov 2015 14:41 |
Last Modified: | 01 Feb 2018 04:30 |
Published Version: | http://dx.doi.org/10.1002/sia.5396 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/sia.5396 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:89326 |