Queiroz, DMM, Saito, M, Rocha, GA et al. (7 more authors) (2013) Helicobacter pylori infection in infants and toddlers in South America: concordance between [¹³C] urea breath test and monoclonal H. pylori stool antigen test. Journal of Clinical Microbiology, 51 (11). pp. 3735-3740. ISSN 0095-1137
Abstract
Accurate noninvasive tests for diagnosing Helicobacter pylori infection in very young children are strongly required. We investigated the agreement between the [¹³C] urea breath test ([¹³C]UBT) and a monoclonal ELISA (HpSA) for detection of H. pylori antigen in stool. From October 2007 to July 2011, we enrolled 414 infants (123 from Brazil and 291 from Peru) of ages 6 to 30 months. Breath and stool samples were obtained at intervals of at least 3 months from Brazilian (n = 415) and Peruvian (n = 908) infants. [¹³C]UBT and stool test results concurred with each other in 1,255 (94.86%) cases (kappa coefficient = 0.90; 95% confidence interval [CI] = 0.87 to 0.92). In the H. pylori-positive group, delta-over-baseline (DOB) and optical density (OD) values were positively correlated (r = 0.62; P < 0.001). The positivity of the tests was higher (P < 0.001; odds ratio [OR] = 6.01; 95% CI = 4.50 to 8.04) in Peru (546/878; 62.2%) than in Brazil (81/377; 21.5%) and increased with increasing age in Brazil (P = 0.02), whereas in Peru it decreased with increasing age (P < 0.001). The disagreement between the test results was associated with birth in Brazil and female gender but not with age and diarrhea. Our results suggest that both [¹³C]UBT and the stool monoclonal test are reliable for diagnosing H. pylori infection in very young children, which will facilitate robust epidemiological studies in infants and toddlers.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2013, American Society for Microbiology. All Rights Reserved. |
Keywords: | Antigens, Bacterial; Brazil; Breath Tests; Child, Preschool; Clinical Laboratory Techniques; Enzyme-Linked Immunosorbent Assay; Feces; Female; Helicobacter Infections; Helicobacter pylori; Humans; Infant; Male; Peru; Prevalence; Urease |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Molecular Gastroenterology (Leeds) |
Funding Information: | Funder Grant number European Commission Sixth Framework Programme INCO- DEV Sector. UNSPECIFIED EU - European Union 032126 |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Jul 2016 15:03 |
Last Modified: | 13 Jul 2016 15:12 |
Published Version: | http://doi.org/10.1128/JCM.01752-13 |
Status: | Published |
Publisher: | American Society for Microbiology |
Identification Number: | 10.1128/JCM.01752-13 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:88545 |