Hernandez Gomez, MC, Rydahl, MG, Rogowski, A et al. (8 more authors) (2015) Recognition of xyloglucan by the crystalline cellulose-binding site of a family 3a carbohydrate-binding module. FEBS Letters, 589 (18). pp. 2297-2303. ISSN 0014-5793
Abstract
Type A non-catalytic carbohydrate-binding modules (CBMs), exemplified by CtCBM3acipA, are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble b-1,4-glucan containing a-1,6-xylose side chains. CtCBM3acipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3acipA. A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM-polysaccharide interactions is discussed.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Keywords: | Carbohydrate-binding module; CBM3a; Xyloglucan; Crystalline cellulose; Plant cell wall |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Oct 2016 12:11 |
Last Modified: | 04 Oct 2016 12:11 |
Published Version: | http://dx.doi.org/10.1016/j.febslet.2015.07.009 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.febslet.2015.07.009 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:88423 |