Dallas, S.L., Miyazono, K., Skerry, T.M. orcid.org/0000-0003-1319-5575 et al. (2 more authors) (1995) Dual role for the latent transforming growth factor-beta binding protein in storage of latent TGF-beta in the extracellular matrix and as a structural matrix protein. The Journal of Cell Biology, 131 (2). pp. 539-549. ISSN 0021-9525
Abstract
The role of the latent TGF-beta binding protein (LTBP) is unclear. In cultures of fetal rat calvarial cells, which form mineralized bonelike nodules, both LTBP and the TGF-beta 1 precursor localized to large fibrillar structures in the extracellular matrix. The appearance of these fibrillar structures preceded the appearance of type I collagen fibers. Plasmin treatment abolished the fibrillar staining pattern for LTBP and released a complex containing both LTBP and TGF-beta. Antibodies and antisense oligonucleotides against LTBP inhibited the formation of mineralized bonelike nodules in long-term fetal rat calvarial cultures. Immunohistochemistry of fetal and adult rat bone confirmed a fibrillar staining pattern for LTBP in vivo. These findings, together with the known homology of LTBP to the fibrillin family of proteins, suggest a novel function for LTBP, in addition to its role in matrix storage of latent TGF-beta, as a structural matrix protein that may play a role in bone formation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 1995 The Rockefeller University Press. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | Animals Bone Development Bone Matrix; metabolism Carrier Proteins/analysis; metabolism Cells, Cultured Extracellular Matrix; metabolism Immunohistochemistry; Intracellular Signaling Peptides and Proteins Latent TGF-beta Binding Proteins Rats Skull embryology; metabolism Transforming Growth Factor beta analysis; metabolism |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 28 Sep 2016 09:34 |
Last Modified: | 28 Sep 2016 09:34 |
Published Version: | http://dx.doi.org/10.1083/jcb.131.2.539 |
Status: | Published |
Publisher: | Rockefeller University Press |
Refereed: | Yes |
Identification Number: | 10.1083/jcb.131.2.539 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:88300 |