Husnain, S.I. and Thomas, M.S. (2008) Downregulation of the Escherichia coli guaB promoter by FIS. Microbiology, 154 (6). 1729 - 1738. ISSN 1350-0872
Abstract
The Escherichia coli guaB promoter (PguaB) regulates transcription of two genes, guaB and guaA, that are required for the synthesis of guanosine 59-monophosphate (GMP), a precursor for the synthesis of guanine nucleoside triphosphates. Transcription from PguaB increases as a function of increasing cellular growth rate, and this is referred to as growth rate-dependent control (GRDC). Here we investigated the role of the factor for inversion stimulation (FIS) in the regulation of this promoter. The results showed that there are three binding sites for FIS centred near positions ”11, +8 and +29 relative to the guaB transcription start site. Binding of FIS to these sites results in repression of PguaB in vitro but not in vivo. Deletion of the fis gene results in increased PguaB activity in vivo, but GRDC of PguaB is maintained.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2008. Seyyed I. Husnain and Mark S. Thomas . This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | RIBOSOMAL-RNA TRANSCRIPTION; RATE-DEPENDENT CONTROL; FACTOR-INDEPENDENT ACTIVATION; GROWTH-RATE REGULATION; RRNB P1; NUCLEOPROTEIN COMPLEXES; BACTERIAL PROMOTERS; NUCLEOID PROTEINS; SUBUNIT LINKER; BINDING-SITES |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 29 Jul 2015 10:17 |
Last Modified: | 29 Jul 2015 10:17 |
Published Version: | http://dx.doi.org/10.1099/mic.0.2008/016774-0 |
Status: | Published |
Publisher: | Society for General Microbiology |
Refereed: | Yes |
Identification Number: | 10.1099/mic.0.2008/016774-0 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:88268 |