Trantidou, T, Terracciano, CM, Kontziampasis, D et al. (2 more authors) (2015) Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials. Scientific reports, 5. 11067 - 11067. ISSN 2045-2322 (Submitted)
Abstract
Current platforms for in vitro drug development utilize confluent, unorganized monolayers of heart cells to study the effect on action potential propagation. However, standard cell cultures are of limited use in cardiac research, as they do not preserve important structural and functional properties of the myocardium. Here we present a method to integrate a scaffolding technology with multi-electrode arrays and deliver a compact, off-the-shelf monitoring platform for growing biomimetic cardiac tissue. Our approach produces anisotropic cultures with conduction velocity (CV) profiles that closer resemble native heart tissue; the fastest impulse propagation is along the long axis of the aligned cardiomyocytes (CVL) and the slowest propagation is perpendicular (CVT), in contrast to standard cultures where action potential propagates isotropically (CVL ≈ CVT). The corresponding anisotropy velocity ratios (CVL/CVT = 1.38 - 2.22) are comparable with values for healthy adult rat ventricles (1.98 - 3.63). The main advantages of this approach are that (i) it provides ultimate pattern control, (ii) it is compatible with automated manufacturing steps and (iii) it is utilized through standard cell culturing protocols. Our platform is compatible with existing read-out equipment and comprises a prompt method for more reliable CV studies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015. Author (s) This work is licensed under a Creative Commons Attribution 4.0 International License. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) > Institute for Particle Science and Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 25 Jun 2015 14:45 |
Last Modified: | 25 Jun 2015 14:45 |
Published Version: | http://dx.doi.org/10.1038/srep11067 |
Status: | Submitted |
Publisher: | Nature Publishing Group |
Identification Number: | 10.1038/srep11067 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:87378 |