Yang, L., Yaseen, M., Zhao, X. et al. (6 more authors) (2015) Gelatin modified ultrathin silk fibroin films for enhanced proliferation of cells. Biomedical Materials, 10 (2). ISSN 1748-6041
Abstract
Silk fibroin (SF) films were modified with gelatin (G) to explore if such SF/G films could enhance the surface biocompatibility of silk as cell growth biomaterials. Ultrathin films were coated from aqueous SF solutions pre-mixed with different amounts of G. It was found that the SF/G blended films after methanol treatment were highly stable in physiological conditions. The incorporation of G smoothed the surface morphology of the SF/G films formed. Surface-exposed RGD sequences were successfully identified on the SF/G films through specific recognition of an integrin-mimicking peptide (bearing the sequence of CWDDGWLC). Cell culture experiments with 3T3 fibroblasts demonstrated that SF/G films with 1.2-20% (w/w) G gave clear improvement in promoting cell attachment and proliferation over pure SF films. Films containing 10-20% (w/w) of G showed cell attachment and growth even superior to the pure G films. The differences as observed from this study suggest that due to the lack of mechanical strength associated with its high solubility, G could not work alone as a cell growth scaffold. The enhanced cellular responses from the blended SF/G films must result from improvement in film stability arising from SF and in cytocompatibility arising from G. The results thus indicate the potential of the SF/G blends in tissue engineering and biomedical engineering where physical and biological properties could be manipulated via mixing either as bulk biomaterials or for coating purposes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 IOP Publishing Ltd. This is an author produced version of a paper subsequently published in Biomedical Materials. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Silk fibroin; Gelatin; Ultrathin films; Film stability; Biocompatibility; RGD; Cell attachment; Proliferation |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Oct 2015 14:44 |
Last Modified: | 18 Nov 2016 19:54 |
Published Version: | http://dx.doi.org/10.1088/1748-6041/10/2/025003 |
Status: | Published |
Publisher: | IOP Publishing |
Refereed: | Yes |
Identification Number: | 10.1088/1748-6041/10/2/025003 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:86323 |