Ansell, RJ (2015) Characterization of the Binding Properties of Molecularly Imprinted Polymers. In: Mattiasson, B and Ye, L, (eds.) Molecularly Imprinted Polymers in Biotechnology. Advances in Biochemical Engineering/Biotechnology, 150 . Springer International Publishing , 51 - 93. ISBN 978-3-319-20728-5
Abstract
The defining characteristic of the binding sites of any particular molecularly imprinted material is heterogeneity: that is, they are not all identical. Nonetheless, it is useful to study their fundamental binding properties, and to obtain average properties. In particular, it has been instructive to compare the binding properties of imprinted and non-imprinted materials. This chapter begins by considering the origins of this site heterogeneity. Next, the properties of interest of imprinted binding sites are described in brief: affinity, selectivity, and kinetics. The binding/adsorption isotherm, the graph of concentration of analyte bound to a MIP versus concentration of free analyte at equilibrium, over a range of total concentrations, is described in some detail. Following this, the techniques for studying the imprinted sites are described (batch-binding assays, radioligand binding assays, zonal chromatography, frontal chromatography, calorimetry, and others). Thereafter, the parameters that influence affinity, selectivity and kinetics are discussed (solvent, modifiers of organic solvents, pH of aqueous solvents, temperature). Finally, mathematical attempts to fit the adsorption isotherms for imprinted materials, so as to obtain information about the range of binding affinities characterizing the imprinted sites, are summarized.
Metadata
Item Type: | Book Section |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | (c) 2015, Springer International Publishing Switzerland. This is an author produced version of a paper published in Molecular Imprinted Polymers in Biotechnology. The final publication is available at Springer via http://dx.doi.org/10.1007/10_2015_316 |
Keywords: | Batch binding; Binding isotherms; Binding site heterogeneity; Chromatographic analysis; Selectivity |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Physical Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Sep 2015 13:18 |
Last Modified: | 22 Apr 2016 00:23 |
Published Version: | http://dx.doi.org/10.1007/10_2015_316 |
Status: | Published |
Publisher: | Springer International Publishing |
Series Name: | Advances in Biochemical Engineering/Biotechnology |
Identification Number: | 10.1007/10_2015_316 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:85148 |