Lee, JA, Knight, CA, Kun, X et al. (4 more authors) (2015) In vivo biocompatibility of custom-fabricated apatite-wollastonite-mesenchymal stromal cell constructs. Journal of biomedical materials research. Part A. ISSN 1549-3296
Abstract
We have used the additive manufacturing technology of selective laser sintering (SLS), together with post SLS heat treatment, to produce porous three dimensional scaffolds from the glass-ceramic apatite-wollastonite (A-W). The A-W scaffolds were custom-designed to incorporate a cylindrical central channel to increase cell penetration and medium flow to the centre of the scaffolds under dynamic culture conditions during in vitro testing and subsequent in vivo implantation. The scaffolds were seeded with human bone marrow mesenchymal stromal cells (MSCs) and cultured in spinner flasks. Using confocal and scanning electron microscopy, we demonstrated that MSCs formed and maintained a confluent layer of viable cells on all surfaces of the A-W scaffolds during dynamic culture. MSC-seeded, with and without osteogenic pre-differentiation, and unseeded A-W scaffolds were implanted subcutaneously in MF1 nude mice where osteoid formation and tissue in-growth were observed following histological assessment. The results demonstrate that the in vivo biocompatibility and osteo-supportive capacity of A-W scaffolds can be enhanced by SLS-custom design, without the requirement for osteogenic pre-induction, to advance their potential as patient-specific bone replacement materials.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is the peer reviewed version of the following article: Lee, JA, Knight, CA, Kun, X, Yang, XB, Wood, DJ, Dalgarno, KW and Genever, PG (2015) In vivo biocompatibility of custom-fabricated apatite-wollastonite-mesenchymal stromal cell constructs. Journal of biomedical materials research.which has been published in final form at http://dx.doi.org/10.1002/jbm.a.35448. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
Keywords: | Apatite-wollastonite; Glass-ceramic; Selective laser sintering; Mesenchymal stromal cells; In vivo biocompatibility |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 May 2015 15:10 |
Last Modified: | 01 Apr 2016 11:34 |
Published Version: | http://dx.doi.org/10.1002/jbm.a.35448 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/jbm.a.35448 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:84530 |