Zaccari, I, Davies, AG, Wälti, C et al. (1 more author) (2015) Label-free electrochemical biosensors for clinical diagnostic. In: Proceedings of the 7th Cairo International Biomedical Engineering Conference, CIBEC 2014. 2014 Cairo International Biomedical Engineering Conference (CIBEC), 11-13 Dec 2014, Giza, Egypt. Institute of Electrical and Electronics Engineers Inc. , 15 - 18. ISBN 9781479944125
Abstract
We present the development of a high sensitivity, label-free, biosensor platform suitable for multiplexed point-of-care diagnostics. A sensor surface based on a carboxy-terminated oligo ethylene-glycol (OEG) self-assembled monolayer (SAM) was developed and fully characterised. Optimal conditions for antibody immobilisation were found for a buffer pH approximately one unit below the pI of the antibody, which yielded both higher antibody density on the sensor surface as well as higher sensor response to the antigen. At the same time the surface showed good resistance to non-specific adsorption of high concentrations of proteins. A non-faradaic electrochemical impedance spectroscopy biosensor to detect human chorionic gonadotropin (hCG) in full serum was demonstrated as a proof of concept. By using the phase of the impedance at 100 mHz as the sensor response, a linear relationship of the phase shift vs the logarithm of hCG concentration was established between 26 fM and 0.26 nM with a sensitivity of 0.6 degree per decade, which is a significant improvement over current state-of-the-art biosensor systems.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2014 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works |
Keywords: | Biosensors; hCG; Impedance Spectroscopy; Label-free |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 May 2015 14:50 |
Last Modified: | 19 Dec 2022 13:31 |
Published Version: | http://dx.doi.org/10.1109/CIBEC.2014.7020946 |
Status: | Published |
Publisher: | Institute of Electrical and Electronics Engineers Inc. |
Identification Number: | 10.1109/CIBEC.2014.7020946 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:84302 |