Allison, T.F., Powles-Glover, N.S., Biga, V. et al. (2 more authors) (2015) Human pluripotent stem cells as tools for high-throughput and high-content screening in drug discovery. International Journal of High Throughput Screening, 5. 1 - 13 . ISSN 1179-1381
Abstract
A significant bottleneck in drug discovery is the lack of suitable models for sensitive, reliable, and rapid assessment of lead molecules in preclinical stages of drug discovery. Human pluripotent stem cells (hPSCs) derived either from early human blastocysts (human embryonic stem cells) or by reprogramming somatic cells to a pluripotent state (human-induced pluripotent stem cells) can be propagated extensively in vitro while retaining the ability to differentiate into any specialized cell type within the body. In this review, we discuss how these unique features of hPSCs could offer a way of producing relevant in vitro models amenable to high-throughput testing for drug discovery. We summarize recent progress in inducing differentiation of hPSCs to specific cell types, and describe the ongoing efforts in applying hPSCs and their differentiated derivatives in disease modeling, drug discovery, and developmental toxicology. Moreover, we review the applications of high-content imaging assays in detecting the changes in the phenotype of hPSCs and their differentiated progeny. Finally, we highlight challenges that need to be overcome in order for the application of hPSC technology to fully benefit drug discovery.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2015 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution - Non Commercial (unported, v3.0) License. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Automatic Control and Systems Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 20 Mar 2015 11:11 |
Last Modified: | 20 Mar 2015 11:11 |
Published Version: | http://dx.doi.org/10.2147/IJHTS.S58113 |
Status: | Published |
Publisher: | Dove Press |
Refereed: | Yes |
Identification Number: | 10.2147/IJHTS.S58113 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:84169 |