Tiernan, JP, Ingram, N, Marston, G et al. (6 more authors) (2015) CEA-targeted nanoparticles allow specific in vivo fluorescent imaging of colorectal cancer models. Nanomedicine. 1 - 9. ISSN 1743-5889
Abstract
Fluorescent imaging of colorectal tumor cells would improve tumor localization and allow intra-operative staging, facilitating stratification of surgical resections thereby improving patient outcomes. We aimed to develop and test fluorescent nanoparticles capable of allowing this in vivo. Dye-doped silica nanoparticles were synthesized. Anti-CEA (carcinoembryonic antigen) or control IgGs were conjugated to nanoparticles using various chemical strategies. Binding of CEA-targeted or control nanoparticles to colorectal cancer cells was quantified in vitro, and in vivo after systemic-delivery to murine xenografts. CEA-targeted, polyamidoamine dendrimer-conjugated, nanoparticles, but not control nanoparticles, allowed strong tumor-specific imaging. We are the first to demonstrate live, specific, in vivo imaging of colorectal cancer cells using antibody-targeted fluorescent nanoparticles. These nanoparticles have potential to allow intra-operative fluorescent visualization of tumor cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015, Future Medicine Ltd. This is an author produced version of a paper published in Nanomedicine. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Colorectal cancer; dye-doped; IVIS imaging; live tumor imaging; silica nanoparticles; tumor markers |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Inst of Biomed & Clin Sciences (LIBACS) (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 Mar 2015 12:12 |
Last Modified: | 16 Nov 2016 16:18 |
Published Version: | http://dx.doi.org/10.2217/NNM.14.202 |
Status: | Published |
Publisher: | Future Medicine Ltd |
Refereed: | Yes |
Identification Number: | 10.2217/NNM.14.202 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:84085 |