Lo Leggio, Leila, Simmons, Thomas J., Poulsen, Jens Christian N et al. (15 more authors) (2015) Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase. Nature Communications. 5961. ISSN 2041-1723
Abstract
Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by β-amylase. The detailed structure of the enzymes active site yields insights into the mechanism of action of this important class of enzymes.
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Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/L000423/1 |
Depositing User: | Pure (York) |
Date Deposited: | 09 Mar 2015 14:43 |
Last Modified: | 25 Dec 2024 00:12 |
Published Version: | https://doi.org/10.1038/ncomms6961 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/ncomms6961 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:84048 |
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Description: Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase
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