Tonge, P.D., Olariu, V., Coca, D. et al. (4 more authors) (2010) Prepatterning in the Stem Cell Compartment. PLOS ONE, 5 (5). e10901. - . ISSN 1932-6203
Abstract
The mechanism by which an apparently uniform population of cells can generate a heterogeneous population of differentiated derivatives is a fundamental aspect of pluripotent and multipotent stem cell behaviour. One possibility is that the environment and the differentiation cues to which the cells are exposed are not uniform. An alternative, but not mutually exclusive possibility is that the observed heterogeneity arises from the stem cells themselves through the existence of different interconvertible substates that pre-exist before the cells commit to differentiate. We have tested this hypothesis in the case of apparently homogeneous pluripotent human embryonal carcinoma (EC) stem cells, which do not follow a uniform pattern of differentiation when exposed to retinoic acid. Instead, they produce differentiated progeny that include both neuronal and non-neural phenotypes. Our results suggest that pluripotent NTERA2 stem cells oscillate between functionally distinct substates that are primed to select distinct lineages when differentiation is induced.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2010 Tonge et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | NEURONAL DIFFERENTIATION; TERATOCARCINOMA CELLS; INFORMATION CRITERION; LINEAGE-COMMITMENT; NEURAL PRECURSORS; PROGENITOR CELLS; MODEL SELECTION; GLOBO-SERIES; IN-VITRO; PLURIPOTENCY |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Automatic Control and Systems Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 20 Mar 2015 14:18 |
Last Modified: | 20 Mar 2015 14:18 |
Published Version: | http://dx.doi.org/10.1371/journal.pone.0010901 |
Status: | Published |
Publisher: | Public Library of Science |
Refereed: | Yes |
Identification Number: | 10.1371/journal.pone.0010901 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:84013 |