Obembe, OO, Jacobsen, E, Timmers, J et al. (5 more authors) (2007) Promiscuous, non-catalytic, tandem carbohydrate-binding modules modulate the cell-wall structure and development of transgenic tobacco (Nicotiana tabacum) plants. Journal of Plant Research, 120 (5). 605 - 617. ISSN 0918-9440
Abstract
We have compared heterologous expression of two types of carbohydrate binding module (CBM) in tobacco cell walls. These are the promiscuous CBM29 modules (a tandem CBM29-1-2 and its single derivative CBM29-2), derived from a non-catalytic protein1, NCP1, of the Piromyces equi cellulase/hemicellulase complex, and the less promiscuous tandem CBM2b-1-2 from the Cellulomonas fimi xylanase 11A. CBM-labelling studies revealed that CBM29-1-2 binds indiscriminately to every tissue of the wild-type tobacco stem whereas binding of CBM2b-1-2 was restricted to vascular tissue. The promiscuous CBM29-1-2 had much more pronounced effects on transgenic tobacco plants than the less promiscuous CBM2b-1-2. Reduced stem elongation and prolonged juvenility, resulting in delayed flower development, were observed in transformants expressing CBM29-1-2 whereas such growth phenotypes were not observed for CBM2b-1-2 plants. Histological examination and electron microscopy revealed layers of collapsed cortical cells in the stems of CBM29-1-2 plants whereas cellular deformation in the stem cortical cells of CBM2b-1-2 transformants was less severe. Altered cell expansion was also observed in most parts of the CBM29-1-2 stem whereas for the CBM2b-1-2 stem this was observed in the xylem cells only. The cellulose content of the transgenic plants was not altered. These results support the hypothesis that CBMs can modify cell wall structure leading to modulation of wall loosening and plant growth.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Carbohydrate-binding modules; cell expansion; cell wall modification; cellulose– hemicellulose networks; nicotiana tabacum; plant growth and development; promiscuous polysaccharide recognition |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 Mar 2015 12:33 |
Last Modified: | 24 Oct 2015 21:38 |
Published Version: | http://dx.doi.org/10.1007/s10265-007-0099-7 |
Status: | Published |
Publisher: | Tokyo: Botanical Society of Japan |
Identification Number: | 10.1007/s10265-007-0099-7 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83722 |