Carr, SB, Mecia, LB, Phillips, SEV et al. (1 more author) (2013) Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69 (10). 1123 - 1126. ISSN 1744-3091
Abstract
Antibiotic resistance in bacterial pathogens poses an ever-increasing risk to human health. In antibiotic-resistant strains of Staphylococcus aureus this resistance often resides in extra-chromosomal plasmids, such as those of the pT181 family, which replicate via a rolling-circle mechanism mediated by a plasmid-encoded replication initiation protein. Currently, there is no structural information available for the pT181-family Rep proteins. Here, the crystallization of a catalytically active fragment of a homologous replication initiation protein from the thermophile Geobacillus stearothermophilus responsible for the replication of plasmid pSTK1 is reported. Crystals of the RepSTK1 fragment diffracted to a resolution of 2.5 Å and belonged to space group P212121.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2013, International Union of Crystallography. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | Geobacillus stearothermophilus; plasmid pSTK1; plasmid pT181; replication initiation proteins; rolling-circle replication; Staphylococcus aureus |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 Mar 2015 16:03 |
Last Modified: | 23 Mar 2015 16:03 |
Published Version: | http://dx.doi.org/10.1107/S1744309113023828 |
Status: | Published |
Publisher: | International Union of Crystallography |
Identification Number: | 10.1107/S1744309113023828 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83667 |