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Duval, C, Allan, P, Connell, SDA et al. (3 more authors) (2014) Roles of fibrin α- and γ-chain specific cross-linking by FXIIIa in fibrin structure and function. Thrombosis and Haemostasis, 111 (5). 842 - 850. ISSN 0340-6245
Abstract
Factor XIII is responsible for the cross-linking of fibrin γ-chains in the early stages of clot formation, whilst α-chain cross-linking occurs at a slower rate. Although γ- and α-chain cross-linking was previously shown to contribute to clot stiffness, the role of cross-linking of both chains in determining clot structure is currently unknown. Therefore, the aim of this study was to determine the role of individual α- and γ-chain cross-linking during clot formation, and its effects on clot structure. We made use of a recombinant fibrinogen (γQ398N/Q399N/K406R), which does not allow for γ-chain cross-linking. In the absence of cross-linking, intact D-D interface was shown to play a potential role in fibre appearance time, clot stiffness and elasticity. Cross-linking of the fibrin α-chain played a role in the thickening of the fibrin fibres over time, and decreased lysis rate in the absence of α2-antiplasmin. We also showed that α-chain cross-linking played a role in the timing of fibre appearance, straightening fibres, increasing clot stiffness and reducing clot deformation. Cross-linking of the γ-chain played a role in fibrin fibre appearance time and fibre density. Our results show that α- and γ-chain cross-linking play independent and specific roles in fibrin clot formation and structure.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Clot structure; Factor XIII; Fibrinogen |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Mar 2015 10:56 |
Last Modified: | 30 Jun 2020 14:49 |
Published Version: | http://dx.doi.org/10.1160/TH13-10-0855 |
Status: | Published |
Publisher: | Schattauer GmbH |
Identification Number: | 10.1160/TH13-10-0855 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83631 |
Available Versions of this Item
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Roles of fibrin α- and γ-chain specific cross-linking by FXIIIa in fibrin structure and function. (deposited 19 Jan 2015 12:04)
- Roles of fibrin α- and γ-chain specific cross-linking by FXIIIa in fibrin structure and function. (deposited 13 Mar 2015 10:56) [Currently Displayed]