Stead, MA and Wright, SC (2014) Structures of heterodimeric POZ domains of Miz1/BCL6 and Miz1/NAC1. Acta Crystallographica Section F: Structural Biology Communications, 70 (12). 1591 - 1596. ISSN 2053-230X
Abstract
The POZ domain is an evolutionarily conserved protein-protein interaction domain that is found in approximately 40 mammalian transcription factors. POZ domains mediate both homodimerization and the heteromeric interactions of different POZ-domain transcription factors with each other. Miz1 is a POZ-domain transcription factor that regulates cell-cycle arrest and DNA-damage responses. The activities of Miz1 are altered by its interaction with the POZ-domain transcriptional repressors BCL6 and NAC1, and these interactions have been implicated in tumourigenesis in B-cell lymphomas and in ovarian serous carcinomas that overexpress BCL6 and NAC1, respectively. A strategy for the purification of tethered POZ domains that form forced heterodimers is described, and crystal structures of the heterodimeric POZ domains of Miz1/BCL6 and of Miz1/NAC1 are reported. These structures will be relevant for the design of therapeutics that target POZ-domain interaction interfaces.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | (c) 2014, International Union of Crystallography. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | BTB domain; forced heterodimer; tethered heterodimer; transcriptional repressor; Miz1/BCL6; Miz1/NAC1 |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 Mar 2015 12:33 |
Last Modified: | 18 Jan 2018 05:04 |
Published Version: | http://dx.doi.org/10.1107/S2053230X14023449 |
Status: | Published |
Publisher: | International Union of Crystallography |
Identification Number: | 10.1107/S2053230X14023449 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83472 |