Zhang, Y, Zhou, D and He, J (2014) FRET studies between CdTe capped by small-molecule ligands and fluorescent protein. International Journal of Nanoscience, 13 (5/6). 1460013. ISSN 1793-5350
Abstract
Water-soluble luminescent semiconductor nanocrystals also known as quantum dots (QDs) that have prominent photostability, wide absorption cross sections and tunable narrow emission, have been shown as promising probes in immunoassays. QDs are often used as donors in fluorescence resonance energy transfer (FRET) based sensors using organic dyes or fluorescent proteins as acceptors. Here, the FRET between a QD donor and fluorescent protein acceptors has been studied. The fluorescent protein (FP) mCherry appended with a hexa-histidine-tag could effectively self-assemble onto CdTe to produce small donor-acceptor distances and hence highly efficient FRET (efficiency > 80%) at relatively low FP:CdTe copy numbers (ca.1). Using the Förster dipole–dipole interaction formula, the Förster radius (R0) and respective donor acceptor distances for the CdTe-FP FRET systems have been calculated. The binding constants (Kd) of the QD-FP systems have also been evaluated by the emission spectra.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | FRET; CdTe; quantum dot; fluorescent protein |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Feb 2015 10:29 |
Last Modified: | 30 Jun 2020 14:49 |
Published Version: | http://dx.doi.org/10.1142/S0219581X14600138 |
Status: | Published |
Publisher: | World Scientific Publishing Company |
Identification Number: | 10.1142/S0219581X14600138 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83348 |