Laetitia, P, Haas, CT, Hewitt, R et al. (3 more authors) (2014) Artefactual nanoparticle activation of the inflammasome platform: in vitro evidence with a nano-formed calcium phosphate. Nanomedicine. ISSN 1743-5889
Abstract
To determine whether in vitro experimental conditions dictate cellular activation of the inflammasome by apatitic calcium phosphate nanoparticles. ; The responses of blood-derived primary human cells to in situ-formed apatite were investigated under different experimental conditions to assess the effect of aseptic culture, cell rest and duration of particle exposure. Cell death and particle uptake were assessed, while IL-1β and caspase 1 responses, with and without lipopolysaccharide prestimulation, were evaluated as markers of inflammasome activation. ; Under carefully addressed experimental conditions, apatitic nanoparticles did not induce cell death or engage the inflammasome platform, although both could be triggered through artefacts of experimentation. ; In vitro studies often predict that engineered nanoparticles, such as synthetic apatite, are candidates for inflammasome activation and, hence, are toxic. However, the experimental setting must be very carefully considered as it may promote false-positive outcomes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | apatite; caspase 1; experimental conditions; IL-1β; inflammasome; nanoparticle |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) > Institute for Materials Research (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 Feb 2015 11:51 |
Last Modified: | 26 Apr 2015 03:57 |
Published Version: | http://dx.doi.org/10.2217/NNM.14.58 |
Status: | Published |
Publisher: | Future Medicine |
Identification Number: | 10.2217/NNM.14.58 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83058 |