El-Sherbiny, YM, Holmes, TD, Wetherill, LF et al. (12 more authors) (2015) Controlled infection with a therapeutic virus defines the activation kinetics of human natural killer cells in vivo. Clinical and experimental immunology, 180 (1). pp. 98-107. ISSN 0009-9104
Abstract
Human natural killer (NK) cells play an important role in antiviral immunity. However, studying their activation kinetics during infection is highly problematic. A clinical trial of a therapeutic virus provided an opportunity to study human NK cell activation in vivo in a controlled manner. Ten colorectal cancer patients with liver metastases received between one and five doses of oncolytic reovirus prior to surgical resection of their tumour. NK cell surface expression of the interferon-inducible molecules CD69 and tetherin peaked twenty-four to forty-eight hours post-infection, coincident with a peak of interferon-induced gene expression. The interferon response and NK cell activation were transient, declining by ninety-six hours post-infection. Furthermore, neither NK cell activation nor the interferon response were sustained in patients undergoing multiple rounds of virus treatment. These results show that reovirus modulates human NK cell activity in vivo and suggest that this may contribute to any therapeutic effect of this oncolytic virus. Detection of a single, transient peak of activation, despite multiple treatment rounds, has implications for the design of reovirus-based therapy. Furthermore, our results suggest the existence of a post-infection refractory period when the interferon response and NK cell activation are blunted. This refractory period has previously been observed in animal models and may underlie the enhanced susceptibility to secondary infections that is seen following viral infection.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is the accepted version of the following article: El-Sherbiny, YM, Holmes, TD, Wetherill, LF, Black, EV, Wilson, EB, Phillips, SL, Scott, GB, Adair, RA, Dave, R, Scott, KJ, Morgan, RS, Coffey, M, Toogood, GJ, Melcher, AA and Cook, GP (2014) Controlled infection with a therapeutic virus defines the activation kinetics of human natural killer cells in vivo. Clinical and experimental immunology. ISSN 0009-9104 , which has been published in final form at http://dx.doi.org/10.1111/cei.12562. |
Keywords: | Natural killer cells; Innate immunity; Viral infection; Interferon response; Human infection |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) > Experimental Musculoskeletal Medicine (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 16 Feb 2015 14:35 |
Last Modified: | 11 Mar 2016 11:43 |
Published Version: | http://dx.doi.org/10.1111/cei.12562 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1111/cei.12562 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83021 |