Brend, MYP and Koelle, MR (2010) RSBP-1 is a membrane-targeting subunit required by the G alpha(q)-specific but not the G alpha(o)-specific R7 regulator of G protein signaling in Caenorhabditis elegans. Molecular Biology of the Cell, 21 (2). 232 - 243. ISSN 1059-1524
Abstract
Regulator of G protein signaling (RGS) proteins inhibit G protein signaling by activating Gα GTPase activity, but the mechanisms that regulate RGS activity are not well understood. The mammalian R7 binding protein (R7BP) can interact with all members of the R7 family of RGS proteins, and palmitoylation of R7BP can target R7 RGS proteins to the plasma membrane in cultured cells. However, whether endogenous R7 RGS proteins in neurons require R7BP or membrane localization for function remains unclear. We have identified and knocked out the only apparent R7BP homolog in Caenorhabditis elegans, RSBP-1. Genetic studies show that loss of RSBP-1 phenocopies loss of the R7 RGS protein EAT-16, but does not disrupt function of the related R7 RGS protein EGL-10. Biochemical analyses find that EAT-16 coimmunoprecipitates with RSBP-1 and is predominantly plasma membrane-associated, whereas EGL-10 does not coimmunoprecipitate with RSBP-1 and is not predominantly membrane-associated. Mutating the conserved membrane-targeting sequence in RSBP-1 disrupts both the membrane association and function of EAT-16, demonstrating that membrane targeting by RSBP-1 is essential for EAT-16 activity. Our analysis of endogenous R7 RGS proteins in C. elegans neurons reveals key differences in the functional requirements for membrane targeting between members of this protein family.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2010, The American Society for Cell Biology. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | GTPase accelerating protein; C-elegans; RGS proteins; DEP-domain; anchoring protein; nervous-system; in-vivo; R9AP; complex; family |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > NIHR Clinical Research Network Coordinating Centre (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Jan 2015 09:09 |
Last Modified: | 25 Jan 2018 14:44 |
Published Version: | http://dx.doi.org/10.1091/mbc.E09-07-0642 |
Status: | Published |
Publisher: | American Society for Cell Biology |
Identification Number: | 10.1091/mbc.E09-07-0642 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:81787 |