White, SJ orcid.org/0000-0002-9227-9461, Johnson, SD, Sellick, MA et al. (3 more authors) (2015) The Influence of Two‐Dimensional Organization on Peptide Conformation. Angewandte Chemie International Edition, 54 (3). pp. 974-978. ISSN 0044-8249
Abstract
Molecular crowding plays a significant role in regulating molecular conformation in cellular environments. It is also likely to be important wherever high molecular densities are required, for example in surface‐phase studies, in which molecular densities generally far exceed those observed in solution. Using on‐surface circular dichroism (CD) spectroscopy, we have investigated the structure of a synthetic peptide assembled into a highly packed monolayer. The immobilized peptide undergoes a structural transition between α‐helical and random coil conformation upon changes in pH and ionic concentration, but critically the threshold for conformational change is altered dramatically by molecular crowding within the peptide monolayer. This study highlights the often overlooked role molecular crowding plays in regulating molecular structure and function in surface‐phase studies of biological molecules.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | (c) 2015 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. reproduction in any medium, provided the original work is properly cited. |
Keywords: | Coiled coil; molecular crowding; circular dichroism; peptide conformation; surface analysis |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Biological Chemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Jan 2015 11:57 |
Last Modified: | 20 Jun 2021 08:37 |
Published Version: | http://dx.doi.org/10.1002/anie.201408971 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/anie.201408971 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:81782 |