Ormerod, JOM, Ashrafian, H, Maher, AR et al. (7 more authors) (2011) The role of vascular myoglobin in nitrite-mediated blood vessel relaxation. Cardiovascular Research, 89 (3). 560 - 565. ISSN 0008-6363
Abstract
AimsThis work investigates the role of myoglobin in mediating the vascular relaxation induced by nitrite. Nitrite, previously considered an inert by-product of nitric oxide metabolism, is now believed to play an important role in several areas of pharmacology and physiology. Myoglobin can act as a nitrite reductase in the heart, where it is plentiful, but it is present at a far lower level in vascular smooth muscleindeed, its existence in the vessel wall is controversial. Haem proteins have been postulated to be important in nitrite-induced vasodilation, but the specific role of myoglobin is unknown. The current study was designed to confirm the presence of myoglobin in murine aortic tissue and to test the hypothesis that vascular wall myoglobin is important for nitrite-induced vasodilation.Methods and resultsAortic rings from wild-type and myoglobin knockout mice were challenged with nitrite, before and after exposure to the haem-protein inhibitor carbon monoxide (CO). CO inhibited vasodilation in wild-type rings but not in myoglobin-deficient rings. Restitution of myoglobin using a genetically modified adenovirus both increased vasodilation to nitrite and reinstated the wild-type pattern of response to CO.ConclusionMyoglobin is present in the murine vasculature and contributes significantly to nitrite-induced vasodilation. .
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2010 The Author. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | Myoglobin; Nitric oxide; Nitrite; Vasodilation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Nov 2014 13:00 |
Last Modified: | 21 Nov 2014 13:00 |
Published Version: | http://dx.doi.org/10.1093/cvr/cvq299 |
Status: | Published |
Publisher: | Oxford University Press (OUP) |
Identification Number: | 10.1093/cvr/cvq299 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:81315 |