Lu, W, Ji, K, Kirkham, J et al. (5 more authors) (2014) Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells. Cell and Tissue Research, 356 (1). pp. 97-107. ISSN 0302-766X
Abstract
Translational research in bone tissue engineering is essential for “bench to bedside” patient benefit. However, the ideal combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study is to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 wt% and 40 wt% of Bioglass particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy showed that PDLLA/Bioglass porous scaffolds supported ADSC attachment, growth and osteogenic differentiation, as confirmed by enhanced alkaline phosphatase (ALP) activity. Higher Bioglass content of the PDLLA foams increased ALP activity compared with the PDLLA only group. Extracellular matrix deposition after 8 weeks in the in vitro cultures was evident by Alcian blue/Sirius red staining. In vivo bone formation was assessed by using scaffold/ADSC constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks. Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass constructs compared with the PDLLA only group. Thus, the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass composite scaffold with a high-potential stem cell source (human ADSCs) could be a promising approach for bone regeneration in a clinical setting.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2014, Springer. This is an author produced version of a paper published in Cell and Tissue Research. Uploaded in accordance with the publisher's self-archiving policy. The final publication is available at Springer via http://dx.doi.org/10.1007/s00441-013-1770-z |
Keywords: | Bone tissue engineering; Adipose-derived stem cells; PDLLA/Bioglass® composite; Biodegradable polymers; In vivo |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Dentistry (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Nov 2014 16:58 |
Last Modified: | 03 Mar 2020 15:35 |
Published Version: | http://dx.doi.org/10.1007/s00441-013-1770-z |
Status: | Published |
Publisher: | Springer |
Identification Number: | 10.1007/s00441-013-1770-z |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:81207 |