Scott, JNF, Kupinski, AP, Kirkham, CM et al. (2 more authors) (2014) Tale proteins bind to both active and inactive chromatin. Biochemical Journal, 458 (1). 153 - 158. ISSN 0264-6021
Abstract
TALE (transcription activator-like effector) proteins can be tailored to bind to any DNA sequence of choice and thus are of immense utility for genome editing and the specific delivery of transcription activators. However, to perform these functions, they need to occupy their sites in chromatin. In the present study, we have systematically assessed TALE binding to chromatin substrates and find that in vitro TALEs bind to their target site on nucleosomes at the more accessible entry/exit sites, but not at the nucleosome dyad. We show further that in vivo TALEs bind to transcriptionally repressed chromatin and that transcription increases binding by only 2-fold. These data therefore imply that TALEs are likely to bind to their target in vivo even at inactive loci.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) The Authors Journal compilation (c) 2014 Biochemical Society. This is an author produced version of a paper published in Biochemical Journal. Uploaded in accordance with the publisher's self-archiving policy |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Sep 2014 14:50 |
Last Modified: | 15 Jan 2018 21:26 |
Published Version: | http://dx.doi.org/10.1042/BJ20131327 |
Status: | Published |
Publisher: | Portland Press |
Identification Number: | 10.1042/BJ20131327 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:80452 |