An Evaluation of Linear Model Analysis Techniques for Processing Images of Micro-Circulation Activity
Mayhew, J., Dewen, H.u, Zheng, Ying. et al. (5 more authors)
(1996)
An Evaluation of Linear Model Analysis Techniques for Processing Images of Micro-Circulation Activity.
Research Report.
ACSE Research Report 632
.
Department of Automatic Control and Systems Engineering
Abstract
Sequences of images of the cortical surface can be processed to reveal information about the cortical micro-circulation, regional cerebral blood flow (rCBF) and changes induced by neuronal activity. Images of rat sensory motor cortex and testes were processed using different analysis methodologies. The study examined the generalised linear model (GLM) approach and compared the results with standard signal processing methods including principal component analysis (PCA). The GLM method has been used by Friston (1994) in the analysis of functional magnetic resonance imagery (fMRI) to identify regions of focal activity. We investigated the use of this method to analyse video image data of the modulation of fCBF from rat cortex. The results revealed spatio-temporal variations in rCBF in response to stimulation within local regions of cortex. The advantage of the GLM method is that it arguments ordinary signal processing methods with an estimate of statistical reliability.
Using different wavelengths of illumination reveals different spatial structures with different temporal relationships. In image time series data collected under green and red illumination, a phase difference was found in the low frequency~ 0.1Hz vasomotion oscillation. This phase difference occurred in data from both cortex and testes. A possible explanation of these differences is that the spectral absorption characteristics of the tissue reflect changes in the volume proportions of the different haemoglobin derivatives in interacting with the modulation of the volume of blood known to occur. It is known that nitric oxide is involved in the modulation of the blood and flow and that spectral changes occur as the nitric oxide is scavenged by haemoglobin to produce met-haemoglobin and nitrosyl-haemoglobin differentially produced in arterial and venous blood. It is proposed that the combination of these effects gives rises to the phase differences we detect.
Metadata
Item Type:
Monograph
Authors/Creators:
Mayhew, J.
Dewen, H.u
Zheng, Ying.
Askew, S.
Hou, Yuqian.
Berwick, J.
Coffey, P.
Brown, N.
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