Understanding how the complex molecular architecture of mannan-degrading hydrolases contributes to plant cell wall degradation.

Background: The molecular architectures of cell wall degrading enzymes are complex. Results: The activity of mannanases and esterases and CBM function against cell walls are driven by substrate context. Conclusion: GH26 and GH5 mannanases target soluble and cell wall mannans, respectively, and CBM potentiation is cell wall-dependent. Significance: The context of cell wall polysaccharides drives the molecular architectures of cell wall-degrading enzymes.