Summers, HD, Brown, MR, Holton, MD et al. (5 more authors) (2013) Quantification of nanoparticle dose and vesicular inheritance in proliferating cells. ACS Nano, 7 (7). 6129 - 6137. ISSN 1936-0851
Abstract
Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can be obtained for large cell populations that internalize fluorescent nanoparticles by endocytosis, through calibration of cytometry measurements to transmission electron microscopy data. Low-throughput, high-resolution electron imaging of quantum dots in U-2 OS cells is quantified and correlated with high-throughput, low-resolution optical imaging of the nanoparticle-loaded cells. From the correlated data, we obtain probability distribution functions of vesicles per cell and nanoparticles per vesicle. Sampling of these distributions and comparison to fluorescence intensity histograms from flow cytometry provide the calibration factor required to transform the cytometry metric to total particle dose per cell, the mean value of which is 2.4 million. Use of the probability distribution functions to analyze particle partitioning during cell division indicates that, while vesicle inheritance is near symmetric, highly variable vesicle loading leads to a highly asymmetric particle dose within the daughter cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Cell proliferation; data interpretation, statistical; endocytosis; flow cytometry; microscopy, fluorescence; nanoparticles; transport vesicles |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) > Institute for Materials Research (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 12 May 2014 09:34 |
Last Modified: | 26 Apr 2015 10:22 |
Published Version: | http://dx.doi.org/10.1021/nn4019619 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/nn4019619 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:78745 |