Brackenbury, William J. orcid.org/0000-0001-6882-3351 and Djamgoz, Mustafa B. A. (2007) Nerve growth factor enhances voltage-gated Na+ channel activity and transwell migration in Mat-LyLu rat prostate cancer cell line. Journal of cellular physiology. pp. 602-608. ISSN 0021-9541
Abstract
The highly dynamic nature of voltage-gated Na+ channel (VGSC) expression and its controlling mechanism(s) are not well understood. In this study, we investigated the possible involvement of nerve growth factor (NGF) in regulating VGSC activity in the strongly metastatic Mat-LyLu cell model of rat prostate cancer (PCa). NGF increased peak VGSC current density in a time- and dose-dependent manner. NGF also shifted voltage to peak and the half-activation voltage to more positive potentials, and produced currents with faster kinetics of activation; sensitivity to the VGSC blocker tetrodotoxin (TTX) was not affected. The NGF-induced increase in peak VGSC current density was suppressed by both the pan-trk antagonist K252a, and the protein kinase A (PKA) inhibitor KT5720. NGF did not affect the Nav1.7 mRNA level, but the total VGSC alpha-subunit protein level was upregulated. NGF potentiated the cells' migration in Transwell assays, and this was not affected by TTX. We concluded that NGF upregulated functional VGSC expression in Mat-LyLu cells, with PKA as a signaling intermediate, but enhancement of migration by NGF was independent of VGSC activity.
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Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2006, Wiley-Liss, Inc. This is an author produced version of a paper published in Journal of Cellular Physiology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | DEPENDENT PROTEIN-KINASE,SODIUM-CHANNEL,PC12 CELLS,CYCLIC-AMP,HIPPOCAMPAL-NEURONS,METASTATIC CASCADE,MESSENGER-RNA,IN-VITRO,NEUROTROPHIN ACTION,SURFACE EXPRESSION |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 29 Apr 2014 14:00 |
Last Modified: | 16 Oct 2024 12:08 |
Published Version: | https://doi.org/10.1002/jcp.20846 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1002/jcp.20846 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:78725 |
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