Wnęk, M, Górzny, ML, Ward, MB et al. (5 more authors) (2013) Fabrication and characterization of gold nano-wires templated on virus-like arrays of tobacco mosaic virus coat proteins. Nanotechnology, 24 (2). 025605. ISSN 0957-4484
Abstract
The rod-shaped plant virus tobacco mosaic virus (TMV) is widely used as a nano-fabrication template, and chimeric peptide expression on its major coat protein has extended its potential applications. Here we describe a simple bacterial expression system for production and rapid purification of recombinant chimeric TMV coat protein carrying C-terminal peptide tags. These proteins do not bind TMV RNA or form disks at pH 7. However, they retain the ability to self-assemble into virus-like arrays at acidic pH. C-terminal peptide tags in such arrays are exposed on the protein surface, allowing interaction with target species. We have utilized a C-terminal His-tag to create virus coat protein-templated nano-rods able to bind gold nanoparticles uniformly. These can be transformed into gold nano-wires by deposition of additional gold atoms from solution, followed by thermal annealing. The resistivity of a typical annealed wire created by this approach is significantly less than values reported for other nano-wires made using different bio-templates. This expression construct is therefore a useful additional tool for the creation of chimeric TMV-like nano-rods for bio-templating.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2013 IOP Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 licence. |
Keywords: | Capsid Proteins; Crystallization; Gold; Macromolecular Substances; Materials Testing; Metal Nanoparticles; Molecular Conformation; Molecular Imprinting; Particle Size; Surface Properties; Tobacco Mosaic Virus |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) > Institute for Materials Research (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 25 Apr 2014 08:12 |
Last Modified: | 20 Jan 2018 12:00 |
Published Version: | http://dx.doi.org/10.1088/0957-4484/24/2/025605 |
Status: | Published |
Publisher: | IOP Publishing |
Identification Number: | 10.1088/0957-4484/24/2/025605 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:78514 |