Wong, CC and Williamson, G (2013) Inhibition of hydroxycinnamic acid sulfation by flavonoids and their conjugated metabolites. BioFactors, 39 (6). 644 - 651. ISSN 0951-6433
Abstract
Hydroxycinnamic acids and flavonoids are dietary phenolic antioxidants that are abundant in our diet. Hydroxycinnamic acids are highly sulfated in vivo, and sulfotransferases (SULTs), in particular SULT1A1, play a major role in their metabolism. Flavonoids are potent inhibitors of human SULTs. In this study, the potential metabolic interaction between dietary hydroxycinnamic acids and flavonoids was investigated. Flavonoids, such as luteolin, quercetin, daidzein, and genistein, are identified as potent inhibitors of hydroxycinnamic acid sulfation in human liver S9 homogenate with IC values <1 μM. The inhibitory activity was less potent in the human intestinal S9 homogenate. We also demonstrate that quercetin conjugates found in vivo (quercetin-3-O-glucuronide, quercetin-7-O-glucuronide, and quercetin-3'-O-sulfate) moderately inhibited the sulfation of hydroxycinnamic acids in human liver S9. In an intact cellular system, human HepG2 cells, caffeic acid and ferulic acid sulfation was inhibited by luteolin and quercetin (IC: 1.6-3.9 μM). Quercetin-3'-O-sulfate weakly inhibited sulfation. Quercetin glucuronides, limited by their low cellular uptake, were ineffective. These data suggest that the inhibition of SULTs by flavonoids and in vivo flavonoid conjugates may modify the bioavailability of dietary hydroxycinnamic acids by suppressing their conversion to sulfated metabolites.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2013, Wiley. This is an author produced version of a paper published in BioFactors. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Hydroxycinnamic acids; flavonoids; flavonoid conjugates; sulfation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 Apr 2014 10:43 |
Last Modified: | 01 Dec 2014 01:38 |
Published Version: | http://dx.doi.org/10.1002/biof.1127 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/biof.1127 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:78309 |