MacDougall, DA and Calaghan, S (2013) A novel approach to the Langendorff technique: preparation of isolated cardiomyocytes and myocardial samples from the same rat heart. Experimental Physiology, 98 (8). 1295 - 1300. ISSN 0958-0670
Abstract
Researchers in biomedical sciences must continually re-evaluate their investment in experiments using laboratory animals. Our group is interested in various signalling pathways that underlie physiological and pathophysiological functioning of the mammalian heart. Two important resources for this type of work are isolated cardiomyocytes and homogenized or preserved sections of whole myocardium. In order to improve our experimental approach ethically, we devised an adaptation of the Langendorff whole-heart retrograde perfusion technique that allows the isolation of adult rat ventricular cardiomyocytes and processing/storage of myocardium from the same heart. This could result in a 50% reduction in the number of animals required for certain experiments. We believe that a generalized adoption of this method would represent a meaningful reduction of animal use in our field of research and, furthermore, strengthen data sets by permitting correlation between myocyte function and various parameters of myocardial biochemistry/structure in individual hearts. This approach is of particular relevance for studies of cardiac pathology, given the cost and time involved in generating experimental disease models.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2012, Wiley. This is an author produced version of a paper published in Experimental Physiology. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Jan 2015 12:46 |
Last Modified: | 16 Jan 2018 00:27 |
Published Version: | http://dx.doi.org/10.1113/expphysiol.2013.072827 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1113/expphysiol.2013.072827 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:77880 |