Weiss, SA and Jeuken, LJC (2009) Electrodes modified with lipid membranes to study quinone oxidoreductases. Biochemical Society Transactions, 37. 707 - 712. ISSN 0300-5127
Abstract
Quinone oxidoreductases are a class of membrane enzymes that catalyse the oxidation or reduction of membrane-bound quinols/quinones. The conversion of quinone/quinol by these enzymes is difficult to study because of the hydrophobic nature of the enzymes and their substrates. We describe some biochemical properties of quinones and quinone oxidoreductases and then look in more detail at two model membranes that can be used to study quinone oxidoreductases in a native-like membrane environment with their native lipophilic quinone substrates. The results obtained with these model membranes are compared with classical enzyme assays that use water-soluble quinone analogues.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2009, Portland Press. This is an author produced version of a paper published in Biochemical Society Transactions. Uploaded in accordance with the publisher's self-archiving policy. The final version of record is available at http://dx.doi.org/10.1042/BST0370707 |
Keywords: | coenzyme Q; enzyme kinetics; oxidative phosphorylation; protein-film voltammetry; solid-supported bilayer lipid membrane; ubiquinone; Lateral Diffusion-Coefficients; Aerobic Respiratory-Chain; Mitochondrial Complex-I; Modified Gold Electrode; Escherichia-Coli; Ubiquinol Oxidase; Cytochrome BO(3); Coenzyme-Q; Supported Membranes; Ligand-Binding |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 22 Nov 2013 11:19 |
Last Modified: | 15 Sep 2014 02:42 |
Published Version: | http://dx.doi.org/10.1042/BST0370707 |
Status: | Published |
Publisher: | Portland Press |
Identification Number: | 10.1042/BST0370707 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:77058 |