Jeuken, LJC (2008) AFM study on the electric-field effects on supported bilayer lipid membranes. Biophysical Journal, 94 (12). 4711 - 4717. ISSN 0006-3495
Abstract
Electric-field induced changes in structure and conductivity of supported bilayer lipid membranes (SLM) have been studied at submicroscopic resolution using atomic force microscopy and electrochemical impedance spectroscopy. The SLMs are formed on gold surfaces modified with mixed self-assembled monolayers of a cholesterol-tether and 6-mercaptohexanol. At applied potentials of ≤-0.25 V versus standard hydrogen electrode, the conductance of the SLM increases and membrane areas of <150 nm in size are found to elevate from the surface up to 15 nm in height. To estimate the electric field experienced by the lipid membrane, electrowetting has been used to determine the point of zero charge of a 6-mercaptohexanol-modified surface (0.19 6 ± 0.13 V versus standard hydrogen electrode). The effects of electric fields on the structure and conductance of supported membranes are discussed.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2008, Biophysical Society. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | Self-Assembled Monolayers; Atomic-Force Microscope; Biological-Membranes; Molecular-Dynamics; Phase-Separation; Cell-Membranes; Pore Formation; Electroporation; Gold; Simulation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Nov 2013 17:11 |
Last Modified: | 15 Sep 2014 02:43 |
Published Version: | http://dx.doi.org/10.1529/biophysj.107.122887 |
Status: | Published |
Publisher: | Biophysical Society |
Identification Number: | 10.1529/biophysj.107.122887 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:77054 |