Kong, Y, Chen, J, Gao, F et al. (6 more authors) (2013) Near-Infrared Fluorescent Ribonuclease-A-Encapsulated Gold Nanoclusters: Preparation, Characterization, Cancer Cell Targeting and Imaging. Nanoscale, 5 (3). 1009 - 1017 (9). ISSN 2040-3364
Abstract
Ultra-small gold nanoclusters (AuNCs) have unique size-dependent optical, electrical and chemical properties. They have emerged as a new nanomaterial with broad applications in optoelectronics, catalysis, biosensing, and bioimaging. Several strategies have been exploited to prepare AuNCs of different “magic number” sizes, using different templates e.g. dendrimers, polyethyleneimines, peptides, and more recently, proteins. Notwithstanding, almost all bio-template-protected AuNCs reported so far exhibit fairly low fluorescence quantum yields (QYs), typically < 5%, which is especially true for AuNCs prepared using the protein templates. In this paper, we report a facile, one-pot aqueous synthesis of highly fluorescent AuNCs using bovine pancreatic ribonuclease A (RNase-A) as the bio-template. The as-prepared AuNCs not only fluoresce strongly at the near-infrared (NIR) region (λEM = 682 nm), but also exhibit an elevated QY of 12.1%. In addition, the RNase-A-encapsulated AuNC (RNase-A-AuNC) displays an exceptionally large Stokes shift of ~210 nm as well as a single dominant fluorescence lifetime of ~1.5 μs, about three orders of magnitudes longer than biological autofluorescence. Furthermore, by coupling vitamin B12 (VB12) to the RNase-A-AuNC, we develop a multifunctional nanoplatform that is suitable for simultaneous targeting and imaging cancer at the cellular level using Caco-2 cell lines as an in vitro model. Since VB12 has effective uptake pathways in the digestion system, this nanoplatform may have potential for targeted oral drug delivery in vivo.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Keywords: | Gold nanocluster, fluorescence imaging, RNase A, Cancer targeting |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > University of Leeds Research Centres and Institutes > Astbury Centre for Structural Molecular Biology (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 27 Sep 2013 10:58 |
Last Modified: | 23 Jun 2023 21:33 |
Published Version: | http://dx.doi.org/10.1039/C2NR32760K |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/C2NR32760K |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:75684 |