Cheetham, MR, Bramble, JP, McMillan, DGG et al. (4 more authors) (2012) Manipulation and sorting of membrane proteins using patterned diffusion-aided ratchets with AC fields in supported lipid bilayers. Soft Matter, 8 (20). 5459 - 5465 . ISSN 1744-683X
Abstract
We present ratchets capable of directing the movement of charged components within supported bilayer lipid membranes. These ratchets make use of asymmetrically patterned features and AC electric fields, and have been demonstrated to transport charged species such as lipids and transmembrane proteins between two reservoirs. Proteins were present in both orientations in the membrane, with only those with their extra-membranous domain orientated away from the glass substrate being mobile. Proteins in the mobile orientation were transported using these ratchets, thereby sorting the two orientations from one another, and creating an area of the membrane containing five times more protein in one orientation than the other.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2012, Royal Society of Chemistry. This is an author produced version of a paper published in Soft Matter. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | AC electric field, AC field, Charged components, Charged species, Glass substrates, Membrane proteins, Patterned features, Supported bilayer lipid membrane, Supported lipid bilayers, Trans-membrane proteins |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 May 2013 10:34 |
Last Modified: | 29 Mar 2018 17:36 |
Published Version: | http://dx.doi.org/10.1039/c2sm25473e |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/c2sm25473e |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:75509 |