Horan, NJ, Siddiqui, Z and Salter, M (2012) Microstructure and molecular microbiology of rapidly formed hydrogen and methane producing granules in phase-separated anaerobic environments. Engineering and Life Science, 13 (2). 132 - 139 . ISSN 1618-0240
Abstract
Hydrogen and methane were simultaneously produced in a two-phase reactor, op- erated to separate the reactions of hydrogen and methanogen production. Each reactor was inoculated with a seed enriched with different microbial consortia. The first phase was operated with a hydraulic retention time of 7 days and at an organic loading rate of 7.7 g VS L−1 d−1 that produced a stable pH of 5.5. This suppressed the growth of methanogens and as a result, the off gas contained up to 27% hydrogen. The second phase was operated with a hydraulic retention time of 12 days and at an organic loading rate of 3.6 g VS L−1 d−1. This permitted the growth of hydrogenotrophs and methanogens to produce methane at a concen- tration of 60%. Examination of the microbial population of the two reactors both microscopically and using PCR, showed an effective separation of hydrogen- and methane-producing microbial communities. The study revealed that the suppression of hydrogentrophs and methanogens can be achieved by adopting rapid method that leads the growth of hydrogen- and methane-producing granules in phase-separated anaerobic environment.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Bioenergy, Hydrogenotrophs, Methanogens, Mixed populations, Two-phase reactor |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Civil Engineering (Leeds) > Inst for Pathogen Control Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 25 Mar 2013 15:21 |
Last Modified: | 04 Nov 2016 03:33 |
Published Version: | http://dx.doi.org/10.1002/elsc.201200048 |
Status: | Published |
Publisher: | Wiley-VCH Verlag Berlin |
Identification Number: | 10.1002/elsc.201200048 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:75283 |