Conroy, DJR, Millner, PA, Stewart, DI et al. (1 more author) (2010) Biosensing for the environment and defence: aqueous uranyl detection using bacterial surface layer proteins. Sensors, 10 (5). 4739 - 4755 . ISSN 1424-8220
Abstract
The fabrication of novel uranyl (UO22+) binding protein based sensors is reported. The new biosensor responds to picomolar levels of aqueous uranyl ions within minutes using Lysinibacillus sphaericus JG-A12 S-layer protein tethered to gold electrodes. In comparison to traditional self assembled monolayer based biosensors the porous bioconjugated layer gave greater stability, longer electrode life span and a denser protein layer. Biosensors responded specifically to UO22+ ions and showed minor interference from Ni2+, Cs+, Cd2+ and Co2+. Chemical modification of JG-A12 protein phosphate and carboxyl groups prevented UO22+ binding, showing that both moieties are involved in the recognition to UO22+.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2010 Conroy et al; licensee MDPI. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | S-layer, surface layer, protein biosensor, uranium, uranyl, metal ion, sequestering, impedance spectroscopy, self-assembled monolayers, electrochemical impedance spectroscopy, metal-binding proteins, gold electrodes, ions, immunosensors, uranium, acid |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > Institute of Membrane and Systems Biology (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Civil Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Feb 2013 11:35 |
Last Modified: | 24 Oct 2016 18:54 |
Published Version: | http://dx.doi.org/10.3390/s100504739 |
Status: | Published |
Publisher: | MDPI |
Refereed: | Yes |
Identification Number: | 10.3390/s100504739 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:74935 |