Price, H. P., Stark, M. and Smith, D. F. (2007) Trypanosoma brucei ARF1 Plays a Central Role in Endocytosis and Golgi-Lysosome Trafficking. Molecular Biology of the Cell, 18 (3). pp. 864-873. ISSN 1059-1524
Abstract
The ADP ribosylation factor (Arf)1 orthologue in the divergent eukaryote Trypanosoma brucei (Tb) shares characteristics with both Arf1 and Arf6 and has a vital role in intracellular protein trafficking. TbARF1 is Golgi localized in trypanosomes but associates with the plasma membrane when expressed in human cells. Depletion of TbARF1 by RNA interference causes a major decrease in endocytosis, which correlates with Rab5 dissociation from early endosomes. Although the Golgi remains intact, parasites display enlarged flagellar pockets and intracellular flagella. An increase in active GTP-bound TbARF1 in bloodstream parasites is rapidly lethal, correlating with a defect in Golgi-to-lysosome transport. We conclude that the essential Golgi-localizing T. brucei ARF1 has a primary role in the maintenance of both post-Golgi transport and endocytosis and that it is significantly divergent from other characterized ARFs.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | York RAE Import |
Date Deposited: | 16 Mar 2009 16:53 |
Last Modified: | 16 Mar 2009 16:53 |
Published Version: | http://dx.doi.org/10.1091/mbc.E06-08-0736 |
Status: | Published |
Publisher: | American Society for Cell Biology |
Identification Number: | 10.1091/mbc.E06-08-0736 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:7032 |