Khodr, H.H., Hider, R.C. and Duhme-Klair, A.-K. (2002) The iron-binding properties of aminochelin, the mono(catecholamide) siderophore of Azotobacter vinelandii. Journal of Biological Inorganic Chemistry, 7 (7-8). pp. 891-896. ISSN 0949-8257
Abstract
Azotobacter vinelandii produces siderophores with different metal-binding properties, depending on the concentration of Fe(III) and molybdate in the growth medium. The three protonation constants of the mono(catecholamide) siderophore aminochelin were determined by simultaneous spectrophotometric and potentiometric titrations as log K 1=12.1, log K 2=10.22 and log K 3=7.04. Based on the two catechol protonation constants, log K 1 and log K 3, the overall stability constant of the aminochelin iron 3:1 complex was found to be log ß 3=41.3, resulting in a pFe 3+ value of 17.6 at pH 7.45. In order to further investigate the properties of the siderophore, the solubilization of Fe(III) hydroxide by a 8×10 –4 M solution of aminochelin at pH 7 and 25 °C was followed spectrophotometrically in the absence and in the presence of molybdate. It was observed that the addition of molybdate resulted in a significant delay in the solubilization.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Aminochelin ; Azotobacter vinelandii ; Catecholamide ; Siderophore |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | York RAE Import |
Date Deposited: | 29 May 2009 10:31 |
Last Modified: | 29 May 2009 10:31 |
Published Version: | http://dx.doi.org/10.1007/s00775-002-0375-x |
Status: | Published |
Publisher: | Springer Science + Business Media |
Refereed: | Yes |
Identification Number: | 10.1007/s00775-002-0375-x |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:6525 |