Larson, T.R., Edgell, T., Byrne, J. et al. (2 more authors) (2002) Acyl CoA profiles of transgenic plants that accumulate medium-chain fatty acids indicate inefficient storage lipid synthesis in developing oilseeds. The Plant Journal, 32 (4). pp. 519-527. ISSN 0960-7412
Abstract
Several Brassica napus lines transformed with genes responsible for the synthesis of medium- or long-chain fatty acids were examined to determine limiting factor(s) for the subsequent accumulation of these fatty acids in seed lipids. Examination of a decanoic acid (10:0) accumulating line revealed a disproportionately high concentration of 10:0 CoA during seed development compared to long-chain acyl CoAs isolated from the same tissues, suggesting that poor incorporation of 10:0 CoA into seed lipids limits 10:0 fatty acid accumulation. This relationship was also seen for dodecanoyl (12:0) CoA and fatty acid in a high 12:0 line, but not for octadecanoic (18:0) CoA and fatty acid in a high 18:0 line. Comparison of 10:0 CoA and fatty acid proportions from seeds at different developmental stages for transgenic B. napus and Cuphea hookeriana, the source plant for the medium-chain thioesterase and 3-ketoacyl-ACP synthase transgenes, revealed that C. hookeriana incorporates 10:0 CoA into seed lipids more efficiently than transgenic B. napus. Furthermore, β-oxidation and glyoxylate cycle activities were not increased above wild type levels during seed development in the 8:0/10:0 line, suggesting that lipid catabolism was not being induced in response to the elevated 10:0 CoA concentrations. Taken together, these data suggest that transgenic plants that are engineered to synthesize medium-chain fatty acids may lack the necessary mechanisms, such as specific acyltransferases, to incorporate these fatty acids efficiently into seed lipids.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Open access copy available from the journal web site. |
Keywords: | acyl CoA, Brassica napus, beta-oxidation, lipid synthesis, medium-chain fatty acids |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Open Access From Journal |
Date Deposited: | 22 Dec 2008 19:23 |
Last Modified: | 22 Dec 2008 19:23 |
Published Version: | http://dx.doi.org/10.1046/j.1365-313X.2002.01440.x |
Status: | Published |
Publisher: | Wiley-Blackwell |
Refereed: | Yes |
Identification Number: | 10.1046/j.1365-313X.2002.01440.x |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:5095 |