Hogg, K G, Kumkate, S, Anderson, S et al. (1 more author) (2003) Interleukin-12 p40 secretion by cutaneous CD11c(+) and F4/80(+) cells is a major feature of the innate immune response in mice that develop Th1-mediated protective immunity to Schistosoma mansoni. Infection and Immunity. pp. 3563-3571. ISSN 1098-5522
Abstract
Radiation-attenuated (RA) schistosome larvae are potent stimulators of innate immune responses at the skin site of exposure (pinna) that are likely to be important factors in the development of Th1-mediated protective immunity. In addition to causing an influx of neutrophils, macrophages, and dendritic cells (DCs) into the dermis, RA larvae induced a cascade of chemokine and cytokine secretion following in vitro culture of pinna biopsy samples. While macrophage inflammatory protein 1alpha and interleukin-1beta (IL-1beta) were produced transiently within the first few days, the Th1-promoting cytokines IL-12 and IL-18 were secreted at high levels until at least day 14. Assay of C3H/HeJ mice confirmed that IL-12 secretion was not due to lipopolysaccharide contaminants binding Toll-like receptor 4. Significantly, IL-12 p40 secretion was sustained in pinnae from vaccinated mice but not in those from nonprotected infected mice. In contrast, IL-10 was produced from both vaccinated and infected mice. This cytokine regulates IL-12-associated dermal inflammation, since in vaccinated IL-10(-/-) mice, pinna thickness was greatly increased concurrent with elevated levels of IL-12 p40. A significant number of IL-12 p40(+) cells were detected as emigrants from in vitro-cultured pinnae, and most were within a population of rare large granular cells that were Ia(+), consistent with their being antigen-presenting cells. Labeling of IL-12(+) cells for CD11c, CD205, CD8alpha, CD11b, and F4/80 indicated that the majority were myeloid DCs, although a proportion were CD11c(-) F4/80(+), suggesting that macrophages were an additional source of IL-12 in the skin.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2003 American Society for Microbiology |
Keywords: | VACCINE-INDUCED IMMUNITY,DRAINING LYMPH-NODES,CYTOKINE PRODUCTION,LEISHMANIA-MAJOR,DENDRITIC CELLS,IFN-GAMMA,LYMPHOCYTES-T,IN-VITRO,SKIN,MIGRATION |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Sherpa Assistant |
Date Deposited: | 28 Feb 2005 |
Last Modified: | 25 Dec 2024 00:09 |
Published Version: | https://doi.org/10.1128/IAI.71.6.3563-3571.2003 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1128/IAI.71.6.3563-3571.2003 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:308 |