Streamlined Work-Up for High-Throughput Experimentation Using Automated Filtration

Automated pipetting is a valuable tool for the preparation of high-throughput experimentation screens; however, automation in postreaction processing (work-up) steps is often overlooked. Opentrons has created an affordable commercial pipetting robot that unlocks automated liquid dispensing and transferring for even the early-career researcher. This article demonstrates the utility of this affordable automated pipetting solution for postreaction processing through four case studies, selectively sampling biphasic mixtures when applicable. Work-up procedures appropriate for high-throughput screening of academic and industrially relevant reactions, including Suzuki–Miyaura coupling, Buchwald–Hartwig coupling and Miyaura borylation, are described. An additional protocol is designed for determining the appropriate aqueous wash to reduce protodeboronation for both an aryl boronic acid and aryl boronic pinacol ester.